Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
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- Provides center wavelength of 450 nm.
- Intended for fluorescence and absorbance detection.
- Replacement optical filter lens for compatible microplate readers.
- Compatible with select filter-based plate reader models.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Molecular Devices 405 nm filter lens is an optical bandpass filter designed for filter-based microplate readers. It transmits light centered at 405 nm for selective detection in absorbance or fluorescence assays and serves as a replaceable filter accessory compatible with common filter-based readers such as EMax, VMax, UVMax, and ThermoMax models.
- Center wavelength 405 nm.
- Optical bandpass filter lens for filter-based detection.
- Replaceable accessory for microplate readers.
- Suitable for assays requiring detection near 405 nm.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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